Four simple chemometric assisted UV-spectrophotometric methods, classical least square (CLS), inverse least square (ILS), principal component regression (PCR) and partial least squares (PLS) were developed for simultaneous estimation of Lornoxicam (LOR) and Tolperisone Hydrochloride (TPH) in their combined dosage tablet formulation without any chemical separation and any graphical treatment of the overlapping spectra of two drugs. The UV absorption spectra of the studied drugs, in the range of 230–278 nm, showed a considerable degree of spectral overlapping. Beer’s law was obeyed for both drugs in the general concentration ranges of 4–14 µg/ml. Eighteen mixed solutions were prepared for the chemometric calibration as training set and eight mixed solutions were prepared as validation set. The absorbance data matrix was obtained by measuring the absorbance at seventeen wavelength points, from 230 to 278 nm with the interval of 3 nm (Δ = 3 nm). The developed calibrations were successfully tested for laboratory mixtures as well as commercial tablet formulation for their LOR and TPH concentration.
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